spc mouse igg2a Search Results


enac subunits  (StressMarq)
93
StressMarq enac subunits
Enac Subunits, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pmc04167712-169-42-29?v=StressMarq
Average 93 stars, based on 1 article reviews
enac subunits - by Bioz Stars, 2026-08
93/100 stars
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hsp90β  (StressMarq)
93
StressMarq hsp90β
Hsp90β, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/ppr0353917-69-11-13?v=StressMarq
Average 93 stars, based on 1 article reviews
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igg amyloid oligomers stressmarq biosciences cat spc 506 d bi  (StressMarq)
93
StressMarq igg amyloid oligomers stressmarq biosciences cat spc 506 d bi
Igg Amyloid Oligomers Stressmarq Biosciences Cat Spc 506 D Bi, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pmc10905009__mmc1-330-141-144?v=StressMarq
Average 93 stars, based on 1 article reviews
igg amyloid oligomers stressmarq biosciences cat spc 506 d bi - by Bioz Stars, 2026-08
93/100 stars
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mouse anti hsp90 antibody  (StressMarq)
90
StressMarq mouse anti hsp90 antibody
Mouse Anti Hsp90 Antibody, supplied by StressMarq, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pm29561661-118-25-33?v=StressMarq
Average 90 stars, based on 1 article reviews
mouse anti hsp90 antibody - by Bioz Stars, 2026-08
90/100 stars
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spc mouse igg2a  (Novus Biologicals)
93
Novus Biologicals spc mouse igg2a
Spc Mouse Igg2a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/10__1002_slash_admi__202400194-339-68-71?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
spc mouse igg2a - by Bioz Stars, 2026-08
93/100 stars
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rabbit polyclonal igg anti hsp70  (StressMarq)
93
StressMarq rabbit polyclonal igg anti hsp70
Rabbit Polyclonal Igg Anti Hsp70, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pm22531929-186-24-30?v=StressMarq
Average 93 stars, based on 1 article reviews
rabbit polyclonal igg anti hsp70 - by Bioz Stars, 2026-08
93/100 stars
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goat anti rabbit igg apc  (Abcam)
99
Abcam goat anti rabbit igg apc
Goat Anti Rabbit Igg Apc, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pmc06948319-74-16-23?v=Abcam
Average 99 stars, based on 1 article reviews
goat anti rabbit igg apc - by Bioz Stars, 2026-08
99/100 stars
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cl caspase 3  (StressMarq)
90
StressMarq cl caspase 3
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Cl Caspase 3, supplied by StressMarq, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pmc08035014-37-229-230?v=StressMarq
Average 90 stars, based on 1 article reviews
cl caspase 3 - by Bioz Stars, 2026-08
90/100 stars
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mouse control igg  (Vector Laboratories)
96
Vector Laboratories mouse control igg
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Mouse Control Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/bio_rxiv__2020__12__25__424415-398-45-48?v=Vector+Laboratories
Average 96 stars, based on 1 article reviews
mouse control igg - by Bioz Stars, 2026-08
96/100 stars
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goat anti mouse igg  (Abcam)
99
Abcam goat anti mouse igg
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Goat Anti Mouse Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pmc07782204-168-51-56?v=Abcam
Average 99 stars, based on 1 article reviews
goat anti mouse igg - by Bioz Stars, 2026-08
99/100 stars
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mouse igg  (Proteintech)
97
Proteintech mouse igg
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Mouse Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pm40399917-43-72-84?v=Proteintech
Average 97 stars, based on 1 article reviews
mouse igg - by Bioz Stars, 2026-08
97/100 stars
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antibody against flag  (OriGene)
96
OriGene antibody against flag
ZQT selectively induced G-MDSC apoptosis by activating <t>the</t> <t>STAT3/S100A9/Bcl-2/caspase-3</t> signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
Antibody Against Flag, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+mouse+igg2a/pm33732417-69-36-45?v=OriGene
Average 96 stars, based on 1 article reviews
antibody against flag - by Bioz Stars, 2026-08
96/100 stars
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Image Search Results


ZQT selectively induced G-MDSC apoptosis by activating the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Journal: Mediators of Inflammation

Article Title: Ze-Qi-Tang Formula Induces Granulocytic Myeloid-Derived Suppressor Cell Apoptosis via STAT3/S100A9/Bcl-2/Caspase-3 Signaling to Prolong the Survival of Mice with Orthotopic Lung Cancer

doi: 10.1155/2021/8856326

Figure Lengend Snippet: ZQT selectively induced G-MDSC apoptosis by activating the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. (a) MDSCs from the tumor tissue of tumor-bearing mice treated with normal saline, ZQT, STAT3 inhibitor (S3I-201), or ZQT combined with S3I-201 were isolated using the MojoSort Mouse Isolation Kit. The protein expression of Ki67, STAT1, p-STAT1, STAT3, p-STAT3, S100A9, Arg-1, Bcl-2, Bax, cl-PARP, cyt c, and cl-caspase-3 in MDSCs from both normal saline and ZQT-treated mice was confirmed by Western blotting. (b) Lung tumor tissues from normal saline or ZQT-treated tumor-bearing mice were stained using S100A9, Ly6G, Ly6C, CD4, and CD8 antibodies for IHC assay (scale bar: 50 μ M). (c) T-lymphocyte proliferation assay was used to show dose-dependent suppression of T cell proliferation by G-MDSCs isolated from tumor tissue of tumor-bearing mice treated with normal saline or ZQT. CD3 + T cells isolated from tumor stained with 5 μ M CFSE were incubated with G-MDSCs for 72 h. Meanwhile, CD3 and CD28 antibodies were added for CD3 + T cell stimulation. Flow cytometry was used to quantify 72 h CFSE dilution. (d, e) G-MDSCs from tumor tissue of tumor-bearing mice treated with normal saline or ZQT were isolated using flow cytometry. The purity (CD11b + Ly6G + ) was >90% as assessed, and the protein expression of STAT3 and cl-caspase-3 was determined by IF (scale bar: 20 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: The following reagents were used: Matrigel basement membrane matrix (Corning, CAT#356234); Hygromycin B (MK Bio, CAT#MS0003-1G); FBS (fetal bovine serum), PS (penicillin-streptomycin), DMEM (Dulbecco's modified Eagle's medium), Trypsin-EDTA (0.25%) (Thermo Fisher Scientific, CAT#10091148, 15140122, 11995065, and 25200072); XenoLight D-Luciferin-K + Salt Bioluminescent Substrate (PerkinElmer, CAT#122799); Brilliant Violet 510™ anti-mouse CD335 (NKp46), PE/Dazzle™ 594 anti-mouse CD11c, Brilliant Violet 650™ anti-mouse I-A/I-E, Brilliant Violet 421™ anti-mouse F4/80, Alexa Fluor® 700 anti-mouse CD4, Brilliant Violet 605™ anti-mouse CD8a, APC anti-mouse CD8, FITC anti-mouse CD4, PE anti-mouse CD3, FITC anti-mouse CD11b, PE anti-mouse Gr1, APC anti-mouse Ly6G, PerCP5.5 anti-mouse Ly6C, purified anti-mouse CD28, PE-Cy7 anti-mouse CD107 α (LAMP-1) (BioLegend, CAT#137623, 117347, 107641, 123137, 100536, 100743, 100712, 100406, 100205, 101206, 108407, 127614, 128011, 102101, and 121619); Red Cell Lysis (Biosharp, CAT#BL503B); CFSE (Sigma-Aldrich, CAT#21888); EasySep Mouse T Cell Isolation Kit, EasySep Mouse MDSC Isolation Kit (STEMCELL, CAT#19851 and 19867); InVivoMab anti-mouse Ly6G (1A8), InVivoMab rat IgG2a isotype control (Bio X Cell, CAT#BE0075-1 and BE0089); Mouse IFN- γ ELISA Set (BD Biosciences, CAT#555138); STAT1, p-STAT1, STAT3, p-STAT3, S100A9, cl-PARP, Arg-1, Ly6G, Cytochrome c, Cyclin D1 (Cell Signaling Technology, CAT#14994, 58D6, 9139T, 4113S, 73425, 9548S, 93668, 87048, 11940T, and 55506T); 1x TBS (Tris-buffered saline) buffer, Tween 20 (Sangon Biotech, CAT#C508113 and A600560); Immobilon Western Chemiluminescent HRP Substrate (Millipore, CAT# WBKLS0500); rabbit anti-Ki67 antibody (Abcam, CAT#ab16667); anti-Bcl-2 (rabbit) antibody (Rockland Immunochemicals, CAT# 200-401-Z43); purified anti-Bax (Biolegend, CAT#633601); cl-caspase-3 (StressMarq Biosciences Inc., CAT# SPC-1319); DeadEnd Fluorometric TUNEL System (Promega, CAT#REF: G3250); Reactive Oxygen Species Assay Kit (Beyotime, CAT#S0033S), Cell Cycle and Apoptosis Analysis Kit (Beyotime, CAT#S0033S and C1052).

Techniques: Isolation, Expressing, Western Blot, Staining, Lymphocyte Proliferation Assay, Incubation, Cell Stimulation, Flow Cytometry

ZQT induced apoptosis in G-MDSCs in TME and inhibited its immunosuppressive activity by activating the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. Mice were treated with anti-Ly6G neutralizing antibody or isotype control by intraperitoneal injection for 14 days before surgery, after which model mice were given maintenance treatment every other day. (a, b) The bioluminescence images of model mice on day 7 and day 28 after surgery. (c) Survival curves. (d, e) The percentage of G-MDSCs, CD3 + T cells, CD4 + T cells, and CD8 + T cells in tumor tissue were analyzed by flow cytometry. (f, g) CD3 + T cells isolated from the tumor tissue of tumor-bearing C57BL/6 mice in different groups were cocultured with LLC cells for 4 h before they were incubated with CD3, CD8, and CD107 α antibodies. Events shown were finally gated on CD3 and CD8. (h) ELISA analysis for the expression of IFN- γ in cells from tumor tissue. (i) RT-qPCR analysis of Arg-1, iNOS, and S100A9 gene expression and flow cytometry for ROS production. (j) IHC staining for Ly6G and CD8 in tumor tissue (scale bar: 50 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Journal: Mediators of Inflammation

Article Title: Ze-Qi-Tang Formula Induces Granulocytic Myeloid-Derived Suppressor Cell Apoptosis via STAT3/S100A9/Bcl-2/Caspase-3 Signaling to Prolong the Survival of Mice with Orthotopic Lung Cancer

doi: 10.1155/2021/8856326

Figure Lengend Snippet: ZQT induced apoptosis in G-MDSCs in TME and inhibited its immunosuppressive activity by activating the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. Mice were treated with anti-Ly6G neutralizing antibody or isotype control by intraperitoneal injection for 14 days before surgery, after which model mice were given maintenance treatment every other day. (a, b) The bioluminescence images of model mice on day 7 and day 28 after surgery. (c) Survival curves. (d, e) The percentage of G-MDSCs, CD3 + T cells, CD4 + T cells, and CD8 + T cells in tumor tissue were analyzed by flow cytometry. (f, g) CD3 + T cells isolated from the tumor tissue of tumor-bearing C57BL/6 mice in different groups were cocultured with LLC cells for 4 h before they were incubated with CD3, CD8, and CD107 α antibodies. Events shown were finally gated on CD3 and CD8. (h) ELISA analysis for the expression of IFN- γ in cells from tumor tissue. (i) RT-qPCR analysis of Arg-1, iNOS, and S100A9 gene expression and flow cytometry for ROS production. (j) IHC staining for Ly6G and CD8 in tumor tissue (scale bar: 50 μ M). n = 5. Data are expressed as the mean ± SD. n/s: nonstatistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: The following reagents were used: Matrigel basement membrane matrix (Corning, CAT#356234); Hygromycin B (MK Bio, CAT#MS0003-1G); FBS (fetal bovine serum), PS (penicillin-streptomycin), DMEM (Dulbecco's modified Eagle's medium), Trypsin-EDTA (0.25%) (Thermo Fisher Scientific, CAT#10091148, 15140122, 11995065, and 25200072); XenoLight D-Luciferin-K + Salt Bioluminescent Substrate (PerkinElmer, CAT#122799); Brilliant Violet 510™ anti-mouse CD335 (NKp46), PE/Dazzle™ 594 anti-mouse CD11c, Brilliant Violet 650™ anti-mouse I-A/I-E, Brilliant Violet 421™ anti-mouse F4/80, Alexa Fluor® 700 anti-mouse CD4, Brilliant Violet 605™ anti-mouse CD8a, APC anti-mouse CD8, FITC anti-mouse CD4, PE anti-mouse CD3, FITC anti-mouse CD11b, PE anti-mouse Gr1, APC anti-mouse Ly6G, PerCP5.5 anti-mouse Ly6C, purified anti-mouse CD28, PE-Cy7 anti-mouse CD107 α (LAMP-1) (BioLegend, CAT#137623, 117347, 107641, 123137, 100536, 100743, 100712, 100406, 100205, 101206, 108407, 127614, 128011, 102101, and 121619); Red Cell Lysis (Biosharp, CAT#BL503B); CFSE (Sigma-Aldrich, CAT#21888); EasySep Mouse T Cell Isolation Kit, EasySep Mouse MDSC Isolation Kit (STEMCELL, CAT#19851 and 19867); InVivoMab anti-mouse Ly6G (1A8), InVivoMab rat IgG2a isotype control (Bio X Cell, CAT#BE0075-1 and BE0089); Mouse IFN- γ ELISA Set (BD Biosciences, CAT#555138); STAT1, p-STAT1, STAT3, p-STAT3, S100A9, cl-PARP, Arg-1, Ly6G, Cytochrome c, Cyclin D1 (Cell Signaling Technology, CAT#14994, 58D6, 9139T, 4113S, 73425, 9548S, 93668, 87048, 11940T, and 55506T); 1x TBS (Tris-buffered saline) buffer, Tween 20 (Sangon Biotech, CAT#C508113 and A600560); Immobilon Western Chemiluminescent HRP Substrate (Millipore, CAT# WBKLS0500); rabbit anti-Ki67 antibody (Abcam, CAT#ab16667); anti-Bcl-2 (rabbit) antibody (Rockland Immunochemicals, CAT# 200-401-Z43); purified anti-Bax (Biolegend, CAT#633601); cl-caspase-3 (StressMarq Biosciences Inc., CAT# SPC-1319); DeadEnd Fluorometric TUNEL System (Promega, CAT#REF: G3250); Reactive Oxygen Species Assay Kit (Beyotime, CAT#S0033S), Cell Cycle and Apoptosis Analysis Kit (Beyotime, CAT#S0033S and C1052).

Techniques: Activity Assay, Injection, Flow Cytometry, Isolation, Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Immunohistochemistry

The immune regulatory activity of TCM formula ZQT in TME. ZQT induces the apoptosis of G-MDSCs via the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway, resulting in a significant decrease in G-MDSCs and their immunosuppressive activity, consequently promoting the infiltration and killing activity of CD8 + T cells, leading to the inhibition of the proliferation of tumor cells.

Journal: Mediators of Inflammation

Article Title: Ze-Qi-Tang Formula Induces Granulocytic Myeloid-Derived Suppressor Cell Apoptosis via STAT3/S100A9/Bcl-2/Caspase-3 Signaling to Prolong the Survival of Mice with Orthotopic Lung Cancer

doi: 10.1155/2021/8856326

Figure Lengend Snippet: The immune regulatory activity of TCM formula ZQT in TME. ZQT induces the apoptosis of G-MDSCs via the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway, resulting in a significant decrease in G-MDSCs and their immunosuppressive activity, consequently promoting the infiltration and killing activity of CD8 + T cells, leading to the inhibition of the proliferation of tumor cells.

Article Snippet: The following reagents were used: Matrigel basement membrane matrix (Corning, CAT#356234); Hygromycin B (MK Bio, CAT#MS0003-1G); FBS (fetal bovine serum), PS (penicillin-streptomycin), DMEM (Dulbecco's modified Eagle's medium), Trypsin-EDTA (0.25%) (Thermo Fisher Scientific, CAT#10091148, 15140122, 11995065, and 25200072); XenoLight D-Luciferin-K + Salt Bioluminescent Substrate (PerkinElmer, CAT#122799); Brilliant Violet 510™ anti-mouse CD335 (NKp46), PE/Dazzle™ 594 anti-mouse CD11c, Brilliant Violet 650™ anti-mouse I-A/I-E, Brilliant Violet 421™ anti-mouse F4/80, Alexa Fluor® 700 anti-mouse CD4, Brilliant Violet 605™ anti-mouse CD8a, APC anti-mouse CD8, FITC anti-mouse CD4, PE anti-mouse CD3, FITC anti-mouse CD11b, PE anti-mouse Gr1, APC anti-mouse Ly6G, PerCP5.5 anti-mouse Ly6C, purified anti-mouse CD28, PE-Cy7 anti-mouse CD107 α (LAMP-1) (BioLegend, CAT#137623, 117347, 107641, 123137, 100536, 100743, 100712, 100406, 100205, 101206, 108407, 127614, 128011, 102101, and 121619); Red Cell Lysis (Biosharp, CAT#BL503B); CFSE (Sigma-Aldrich, CAT#21888); EasySep Mouse T Cell Isolation Kit, EasySep Mouse MDSC Isolation Kit (STEMCELL, CAT#19851 and 19867); InVivoMab anti-mouse Ly6G (1A8), InVivoMab rat IgG2a isotype control (Bio X Cell, CAT#BE0075-1 and BE0089); Mouse IFN- γ ELISA Set (BD Biosciences, CAT#555138); STAT1, p-STAT1, STAT3, p-STAT3, S100A9, cl-PARP, Arg-1, Ly6G, Cytochrome c, Cyclin D1 (Cell Signaling Technology, CAT#14994, 58D6, 9139T, 4113S, 73425, 9548S, 93668, 87048, 11940T, and 55506T); 1x TBS (Tris-buffered saline) buffer, Tween 20 (Sangon Biotech, CAT#C508113 and A600560); Immobilon Western Chemiluminescent HRP Substrate (Millipore, CAT# WBKLS0500); rabbit anti-Ki67 antibody (Abcam, CAT#ab16667); anti-Bcl-2 (rabbit) antibody (Rockland Immunochemicals, CAT# 200-401-Z43); purified anti-Bax (Biolegend, CAT#633601); cl-caspase-3 (StressMarq Biosciences Inc., CAT# SPC-1319); DeadEnd Fluorometric TUNEL System (Promega, CAT#REF: G3250); Reactive Oxygen Species Assay Kit (Beyotime, CAT#S0033S), Cell Cycle and Apoptosis Analysis Kit (Beyotime, CAT#S0033S and C1052).

Techniques: Activity Assay, Inhibition